Mass spectrometry (MS) is one of the most widely used techniques to characterise proteins and their phosphorylation status in biological and clinical samples. In a typical proteomic workflow, isolated proteins are digested into shorter fragments, known as peptides, which are analysed in a mass spectrometer. A phosphoproteomic workflow is very similar to a conventional bottom-up proteomic workflow with the exception that phosphorylated peptides are typically enriched prior to mass spectrometric acquisition using IMAC (immobilized metal affinity chromatography) or MOAC (metal oxide affinity chromatography). Software packages are then used to identify the (phospho)peptide sequences, quantify their abundance, and specific algorithms merge the peptide information to report protein-level information. Despite the increased utilization of (phospho)proteomics in biomedical research, there is still a lack of simple and user-friendly tools to support downstream analysis and interpretation. This poster presents an automated pipeline to visualise and interrogate phosphoproteomics data in the form of a web-platform called “Phospho-Analyst”. It is an easy to use, interactive web application developed to perform differential expression analysis on both peptide and protein-level data. Phospho-Analyst offers a wealth of analytic features to facilitate statistical, exploratory and comparative analysis of different levels of proteomics datasets. Users can also download results in the form of tables and graphs along with auto-generated reports of key experimental outcomes. Phospho-Analyst can be accessed at https://phosphoanalyst.erc.monash.edu/.