Poster Presentation 28th Annual Lorne Proteomics Symposium 2023

The WEHI Proteomics Facility: tools, techniques and technologies (#110)

Samantha J Emery-Corbin 1 2 , Toby Dite 1 2 , Subash Adhikari 1 2 , Jumana M Yousef 1 2 , Vineet Vaibhav 1 2 , Laura F Dagley 1 2
  1. The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia
  2. Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia

The WEHI Proteomics Facility is a major technology platform integrated within the Advanced Technology & Biology Division providing the latest mass spectrometric instrumentation to facilitate researcher-led exploration, identification and quantification of proteins and peptides. Our team of six experts in mass spectrometry-based proteomics utilise advanced proteomic technologies to facilitate the seamless translation of results to our collaborators. From experimental design and sample preparation, to data analysis and statistics – we offer expertise through all stages of the proteomics workflow. We work collaboratively with both academic researchers both at WEHI and externally, and offer fee-for-service arrangements with commercial organisations.   

Our mission is to provide access to state-of-the-art liquid chromatography mass spectrometry-based proteomic and protein characterisation services with rapid turnaround times. Our four core research interests are methods development, mass spectrometry analysis, statistics & data analysis, and clinical discovery & translation. We facilitate a variety of mass spectrometry-based proteomics experiments including: global proteomics (DIA and DDA), drug-target identification, N-terminomics, post-translational modification (PTM) analyses (including phosphophorylation and ubiquitination), targeted mass spectrometry (PRM/SRM), trotein/recombinant protein identification, intact ‘top-down’ proteomics, cell-surface labelling, TurboID/BioID, crosslinking mass spectrometry (XL-MS) and interactomics (e.g. Co-IP, HA-tagged, FLAG-tag)

Here we provide a detailed outline of our collaborative research services, high resolution instrumentation and highlight our recent achievements in terms of instrument utilisation and method developments.